|Kyle MacLachlan calls his UW actor training 'essential'The Seattle TimesAn interview with actor Kyle MacLachlan (“Twin Peaks,” “Sex and the City,” “Portlandia”), who was the subject of a SIFF tribute this week. He spoke about his screen and TV roles and his University of Washington training. By Moira Macdonald. Seattle …
via “school of aquatic and fishery sciences” – Google News
Evidence for an amoeba-like infectious stage of Ichthyophonus sp. and description of a circulating blood stage: a probable mechanism for dispersal within the fish host.
J Parasitol. 2013 Apr;99(2):235-40
Authors: Kocan R, LaPatra S, Hershberger P
Small amoeboid cells, believed to be the infectious stage of Ichthyophonus sp., were observed in the bolus (stomach contents) and tunica propria (stomach wall) of Pacific staghorn sculpins and rainbow trout shortly after they ingested Ichthyophonus sp.-infected tissues. By 24-48 hr post-exposure (PE) the parasite morphed from the classically reported multinucleate thick walled schizonts to 2 distinct cell types, i.e., a larger multinucleate amoeboid cell surrounded by a narrow translucent zone and a smaller spherical cell surrounded by a “halo” and resembling a small schizont. Both cell types also appeared in the tunica propria, indicating that they had recently penetrated the columnar epithelium of the stomach. No Ichthyophonus sp. pseudo-hyphae (“germination tubes”) were observed in the bolus or penetrating the stomach wall. Simultaneously, Ichthyophonus sp. was isolated in vitro from aortic blood, which was consistently positive from 6 to 144 hr PE, then only intermittently for the next 4 wk. Small PAS-positive cells observed in blood cultures grew into colonies consisting of non-septate tubules (pseudo-hyphae) terminating in multinucleated knob-like apices similar to those seen in organ explant cultures. Organ explants were culture positive every day; however, typical Ichthyophonus sp. schizonts were not observed histologically until 20-25 days PE. From 20 to 60 days PE, schizont diameter increased from ≤ 25 μm to ≥ 82 μm. Based on the data presented herein, we are confident that we have resolved the life cycle of Ichthyophonus sp. within the piscivorous host.
PMID: 22924915 [PubMed - indexed for MEDLINE]
via pubmed: school of aquatic an… http://www.ncbi.nlm.nih.gov/pubmed/22924915?dopt=Abstract
This event will take place at Fish 203 today, June 11, 2013 at 11:30AM. Faculty Meetings are held in FSH 203 from 11:30 AM to 1:00 PM. Faculty Meetings are open to the public, per RCW 42.30 and UW APS 1.5, unless recessed into executive (closed) session.
Pesticides in Urban Streams and Prespawn Mortality of Pacific Coho Salmon.
Arch Environ Contam Toxicol. 2013 Jun 7;
Authors: King KA, Grue CE, Grassley JM, Hearsey JW
The listing of several runs of Pacific salmon as threatened or endangered and associated federal, state, and local efforts to restore/enhance salmon habitat in the Pacific Northwest make it imperative that the factors associated with these population declines are understood. Prespawn mortality (PSM) has been documented in coho salmon (Oncorhynchus kisutch) within urban streams in western Washington since the late 1990s and is characterized by a suite of neurological and respiratory symptoms with mortality occurring shortly thereafter. Mortality rates in returning adults have ranged between 17 and 100 %. The cause of PSM is not known, but the presence of pesticide residues within urban streams led to a hypothesis that PSM in coho salmon and pesticides in urban streams were linked. We exposed pairs of “green” (unripe) prespawn male and female coho salmon to a pesticide mixture (“cocktail”) reported in urban streams in western Washington State, USA. Longevity, ripening in female salmon, and brain acetylcholinesterase were not significantly affected by continuous exposure to the maximum reported concentrations of the pesticides. Fertilization, hatching success, and growth of fry were also not affected when green adults were exposed to these concentrations for 96 h. The absence of effects suggests it is unlikely that pesticides within stormwater are singularly responsible for PSM in coho salmon or that they impair the reproductive capability of exposed adults.
PMID: 23744049 [PubMed - as supplied by publisher]
via pubmed: school of aquatic an… http://www.ncbi.nlm.nih.gov/pubmed/23744049?dopt=Abstract