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Genome-wide profiling of DNA methylation and gene expression in Crassostrea gigas male gametes.

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Genome-wide profiling of DNA methylation and gene expression in Crassostrea gigas male gametes.

Front Physiol. 2014;5:224

Authors: Olson CE, Roberts SB

Abstract
DNA methylation patterns and functions are variable across invertebrate taxa. In order to provide a better understanding of DNA methylation in the Pacific oyster (Crassostrea gigas), we characterized the genome-wide DNA methylation profile in male gamete cells using whole-genome bisulfite sequencing. RNA-Seq analysis was performed to examine the relationship between DNA methylation and transcript expression. Methylation status of over 7.6 million CpG dinucleotides was described with a majority of methylated regions occurring among intragenic regions. Overall, 15% of the CpG dinucleotides were determined to be methylated and the mitochondrial genome lacked DNA methylation. Integrative analysis of DNA methylation and RNA-Seq data revealed a positive association between methylation status, both in gene bodies and putative promoter regions, and expression. This study provides a comprehensive characterization of the distribution of DNA methylation in the oyster male gamete tissue and suggests that DNA methylation is involved in gene regulatory activity.

PMID: 24987376 [PubMed]

via pubmed: school of aquatic an… http://ift.tt/1vMZfYb

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