In association with a research collaboration between UW School of Medicine Dept. of Medical Genetics and University of Osaka Prefecture and also Tohoku University, Japan, surface plasmon resonance (SPR) is being explored as a means of developing a portable multichannel sensor for ciguatoxins (CTXs). As potent enhancers of sodium transport in voltage gated sodium channels (VGSC) ciguatoxins pose a serious threat to human health and the Pacific region forms are so potent that FDA has set advisory levels of 0.01 parts per billion. As of yet the only method capable of detecting such low levels of VGSC effectors uses cultured neuroblastoma cells, now in combination with flow cytometry (Ron Manger et al, FHCRC). Portable surface plasmon resonancedetection has been demonstrated for detecting a wide range of targets from viruses to toxins, and has been demonstrated already for two other marinetoxins by the Clem Furlong group at UW for domoic acid and okadaic acid. These toxins are monitored in Puget Sound shellfish by Washington State Dept of Health. In contrast to these toxins, ciguatoxins are found in in tropical environments, but have also been implicated in outbreaks of ciguatera on
cruise ships and in restaurants.
This mini seminar will include project-relevant talks on SPR by three experts: Clem Furlong, UW Medical Genetics, a discussion of ELISA development for ciguatoxins by Takeshi Tsumuraya of Osaka Prefecture, Japan, and an update on palytoxin and ciguatoxin research by world renowned toxin expert Takeshi Yasumoto, of Japan Food Research Labs, Tokyo.
Acknowledgment – We thank Washington Sea Grant, UW School of Aquatic and Fisheries Sciences, and Pacific Northwest Section, AOAC Int. Please Note: This event is neither promoted nor endorsed by FDA.